Human LIM And SH3 Protein 1 (LASP1) ELISA Kit | LASP1 elisa kit
Human LIM And SH3 Protein 1 (LASP1) ELISA Kit
Gene Names
LASP1; MLN50; Lasp-1
Reactivity
Human
Synonyms
LIM And SH3 Protein 1 (LASP1); N/A; Human LIM And SH3 Protein 1 (LASP1) ELISA Kit; MLN50; Metastatic lymph node gene 50 protein; LASP1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of LASP1. No significant cross-reactivity or interference between LASP1 and analogues was observed.
Sequence Length
205
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.062ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LASP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LASP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for LASP1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LASP1 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LASP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LASP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LASP1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LASP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LASP1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,181 Da
NCBI Official Full Name
LIM and SH3 domain protein 1 isoform a
NCBI Official Synonym Full Names
LIM and SH3 protein 1
NCBI Official Symbol
LASP1
NCBI Official Synonym Symbols
MLN50; Lasp-1
NCBI Protein Information
LIM and SH3 domain protein 1
UniProt Protein Name
LIM and SH3 domain protein 1
UniProt Gene Name
LASP1
UniProt Synonym Gene Names
MLN50; LASP-1; MLN 50
UniProt Entry Name
LASP1_HUMAN
