Human Cytochrome c oxidase subunit 7C, mitochondrial (COX7C) ELISA Kit | COX7C elisa kit

Human Cytochrome c oxidase subunit 7C, mitochondrial (COX7C) ELISA Kit

Cat. Num. AAA27111

• Reactivity: Human
• Synonyms: Cytochrome c oxidase subunit 7C, mitochondrial (COX7C); N/A; Human Cytochrome c oxidase subunit 7C, mitochondrial (COX7C) ELISA Kit; COX7C elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COX7C. No significant cross-reactivity or interference between COX7C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COX7C and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for COX7C elisa kit

Principle of the Assay: COX7C ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COX7C antibody and an COX7C-HRP conjugate. The assay sample and buffer are incubated together with COX7C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX7C concentration since COX7C from samples and COX7C-HRP conjugate compete for the anti-COX7C antibody binding site. Since the number of sites is limited, as more sites are occupied by COX7C from the sample, fewer sites are left to bind COX7C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX7C concentration in each sample is interpolated from this standard curve.

Intended Uses: This COX7C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human COX7C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 4502993
• NCBI GeneID: 1350
• NCBI Accession #: NP_001858.1
• NCBI GenBank Nucleotide #: NM_001867.2
• Molecular Weight: 7,246 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 7C, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit VIIc
• NCBI Official Symbol: COX7C
• NCBI Protein Information: cytochrome c oxidase subunit 7C, mitochondrial; cytochrome-c oxidase chain VIIc; cytochrome c oxidase polypeptide VIIc
• UniProt Protein Name: Cytochrome c oxidase subunit 7C, mitochondrial
• UniProt Gene Name: COX7C
• UniProt Entry Name: COX7C_HUMAN