Rat Carboxypeptidase D (CPD) ELISA Kit | CPD elisa kit

Rat Carboxypeptidase D (CPD) ELISA Kit

Cat. Num. AAA27117

• Gene Names: CPD; GP180
• Reactivity: Rat
• Synonyms: Carboxypeptidase D (CPD); N/A; Rat Carboxypeptidase D (CPD) ELISA Kit; CPD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CPD. No significant cross-reactivity or interference between CPD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPD and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CPD elisa kit

Principle of the Assay: CPD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CPD antibody and an CPD-HRP conjugate. The assay sample and buffer are incubated together with CPD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPD concentration since CPD from samples and CPD-HRP conjugate compete for the anti-CPD antibody binding site. Since the number of sites is limited, as more sites are occupied by CPD from the sample, fewer sites are left to bind CPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPD concentration in each sample is interpolated from this standard curve.

Intended Uses: This CPD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CPD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 22202611
• NCBI GeneID: 1362
• NCBI Accession #: NP_001295.2
• NCBI GenBank Nucleotide #: NM_001304.4
• Molecular Weight: 126,487 Da
• NCBI Official Full Name: carboxypeptidase D isoform 1
• NCBI Official Synonym Full Names: carboxypeptidase D
• NCBI Official Symbol: CPD
• NCBI Official Synonym Symbols: GP180
• NCBI Protein Information: carboxypeptidase D; metallocarboxypeptidase D
• UniProt Protein Name: Carboxypeptidase D
• UniProt Gene Name: CPD
• UniProt Entry Name: CBPD_HUMAN