Human Carboxypeptidase A4 (CPA4) RTU ELISA Kit | CPA4 rtu elisa kit
Human Carboxypeptidase A4 (CPA4) ELISA Kit
Gene Names
CPA4; CPA3
Reactivity
Human
Synonyms
Carboxypeptidase A4 (CPA4); N/A; Human Carboxypeptidase A4 (CPA4) ELISA Kit; CPA4 rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CPA4. No significant cross-reactivity or interference between CPA4 and analogues was observed.
Sequence Length
388
Samples
Serum, plasma, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.051ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPA4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPA4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Standard Curve (Sample)
Related Product Information for CPA4 rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CPA4 in human serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPA4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CPA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPA4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CPA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,570 Da
NCBI Official Full Name
carboxypeptidase A4 isoform 2
NCBI Official Synonym Full Names
carboxypeptidase A4
NCBI Official Symbol
CPA4
NCBI Official Synonym Symbols
CPA3
NCBI Protein Information
carboxypeptidase A4
UniProt Protein Name
Carboxypeptidase A4
UniProt Gene Name
CPA4
UniProt Synonym Gene Names
CPA3
