Mouse Active caspase-3 ELISA Kit | Active caspase-3 elisa kit
Mouse Active caspase-3 ELISA Kit
Reactivity
Mouse
Synonyms
Active caspase-3; N/A; Mouse Active caspase-3 ELISA Kit; Active caspase-3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ACASP3. No significant cross-reactivity or interference between ACASP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACASP3 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for Active caspase-3 elisa kit
Principle of the Assay: ACASP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACASP3 antibody and an ACASP3-HRP conjugate. The assay sample and buffer are incubated together with ACASP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACASP3 concentration since ACASP3 from samples and ACASP3-HRP conjugate compete for the anti-ACASP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACASP3 from the sample, fewer sites are left to bind ACASP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACASP3 concentration in each sample is interpolated from this standard curve.
Intended Uses: This ACASP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACASP3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This ACASP3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACASP3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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