Human ADAMTS like protein 2 (ADAMTSL2) ELISA Kit | ADAMTSL2 elisa kit

Human ADAMTS like protein 2 (ADAMTSL2) ELISA Kit

Cat. Num. AAA27141

• Gene Names: ADAMTSL2; GPHYSD1
• Reactivity: Human
• Synonyms: ADAMTS like protein 2 (ADAMTSL2); N/A; Human ADAMTS like protein 2 (ADAMTSL2) ELISA Kit; ADAMTSL2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADAMTSL2. No significant cross-reactivity or interference between ADAMTSL2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTSL2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ADAMTSL2 elisa kit

Principle of the Assay: ADAMTSL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAMTSL2 antibody and an ADAMTSL2-HRP conjugate. The assay sample and buffer are incubated together with ADAMTSL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTSL2 concentration since ADAMTSL2 from samples and ADAMTSL2-HRP conjugate compete for the anti-ADAMTSL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTSL2 from the sample, fewer sites are left to bind ADAMTSL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTSL2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ADAMTSL2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTSL2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

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Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 223718260
• NCBI GeneID: 9719
• NCBI Accession #: NP_001138792.1
• NCBI GenBank Nucleotide #: NM_001145320.1
• Molecular Weight: 104,621 Da
• NCBI Official Full Name: ADAMTS-like protein 2
• NCBI Official Synonym Full Names: ADAMTS-like 2
• NCBI Official Symbol: ADAMTSL2
• NCBI Official Synonym Symbols: GPHYSD1
• NCBI Protein Information: ADAMTS-like protein 2; ADAMTSL-2
• UniProt Protein Name: ADAMTS-like protein 2
• UniProt Gene Name: ADAMTSL2
• UniProt Synonym Gene Names: KIAA0605; ADAMTSL-2
• UniProt Entry Name: ATL2_HUMAN