Monkey soluble OX40 Ligand ELISA Kit | sOX4L elisa kit

Monkey soluble OX40 Ligand ELISA Kit

Cat. Num. AAA27156

• Reactivity: Monkey
• Synonyms: soluble OX40 Ligand; N/A; Monkey soluble OX40 Ligand ELISA Kit; sOX4L elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of sOX40L. No significant cross-reactivity or interference between sOX40L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sOX40L and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for sOX4L elisa kit

Principle of the Assay: sOX40L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sOX40L antibody and an sOX40L-HRP conjugate. The assay sample and buffer are incubated together with sOX40L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sOX40L concentration since sOX40L from samples and sOX40L-HRP conjugate compete for the anti-sOX40L antibody binding site. Since the number of sites is limited, as more sites are occupied by sOX40L from the sample, fewer sites are left to bind sOX40L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sOX40L concentration in each sample is interpolated from this standard curve.

Intended Uses: This sOX40L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey sOX40L. This ELISA kit for research use only, not for therapeutic or diagnostic applications!