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WB (Western Blot)
(Detection of human OX40/CD134 by western blot. Samples: Whole cell lysate (50 ug) from HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-eEF2 .)
OX40/CD134 recombinant antibody
Rabbit anti-OX40/CD134 Recombinant Monoclonal Antibody [BLR042F]
Gene Names
TNFRSF4; OX40; ACT35; CD134; IMD16; TXGP1L
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
OX40/CD134; Monoclonal Recombinant Antibody; Rabbit anti-OX40/CD134 Recombinant Monoclonal Antibody [BLR042F]; IMD16; tumor necrosis factor receptor superfamily member 4; tax-transcriptionally activated glycoprotein 1 receptor; TAX transcriptionally-activated glycoprotein 1 receptor; OX40L receptor; OX40 homologue; OX40 cell surface antigen; OX40 antigen; TXGP1L; OX40; CD134 antigen; CD134; CD antigen CD134; ATC35 antigen; ACT35 antigen; ACT35; lymphoid activation antigene ACT35; Tumor necrosis factor receptor superfamily member 4; TNF receptor superfamily member 4; OX40/CD134 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR042F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for OX40/CD134 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 4 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 4 ul per 1 x 10^6 cells.
Immunogen
Residues 29-216 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human OX40/CD134 by western blot. Samples: Whole cell lysate (50 ug) from HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-eEF2 .)
WB (Western Blot)
(Detection of human OX40/CD134 by western blot. Samples: Whole cell lysate (50 ug) from HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-eEF2 .)
IP (Immunoprecipitation)
(Detection of human OX40/CD134 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used for IP at 20 ul per reaction. OX40/CD134 was also immunoprecipitated by rabbit anti-OX40/CD134 recombinant monoclonal antibody [548-1D12]. For blotting immunoprecipitated OX40/CD134, AAA23810 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human OX40/CD134 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used for IP at 20 ul per reaction. OX40/CD134 was also immunoprecipitated by rabbit anti-OX40/CD134 recombinant monoclonal antibody [548-1D12]. For blotting immunoprecipitated OX40/CD134, AAA23810 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human OX40/CD134 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human OX40/CD134 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human OX40/CD134 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human OX40/CD134 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human OX40/CD134 by immunocytochemistry. Sample: FFPE section of MJ cells. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human OX40/CD134 by immunocytochemistry. Sample: FFPE section of MJ cells. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (AAA23810 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human OX40/CD134 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal (AAA23810 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human OX40/CD134 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal (AAA23810 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for OX40/CD134 recombinant antibody
OX40/CD134 is a member of the TNF-receptor superfamily that has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested OX40/CD134 plays a role in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. [taken from NCBI Entrez Gene (Gene ID: 3659)].
Product Categories/Family for OX40/CD134 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,341 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 4
NCBI Official Synonym Full Names
TNF receptor superfamily member 4
NCBI Official Symbol
TNFRSF4
NCBI Official Synonym Symbols
OX40; ACT35; CD134; IMD16; TXGP1L
NCBI Protein Information
tumor necrosis factor receptor superfamily member 4
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 4
UniProt Gene Name
TNFRSF4
UniProt Synonym Gene Names
TXGP1L
UniProt Entry Name
TNR4_HUMAN
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Product Notes
The OX40/CD134 tnfrsf4 (Catalog #AAA23810) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-OX40/CD134 Recombinant Monoclonal Antibody [BLR042F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OX40/CD134 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed and permeabilized cells were stained with 4 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the OX40/CD134 tnfrsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OX40/CD134, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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