Canine Carbonic Anhydrase 9 (CA9) ELISA Kit | CA9 elisa kit
Canine Carbonic Anhydrase 9 (CA9) ELISA Kit
Gene Names
CA9; MN; CAIX
Reactivity
Canine
Synonyms
Carbonic Anhydrase 9 (CA9); N/A; Canine Carbonic Anhydrase 9 (CA9) ELISA Kit; CA9 elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of CA9. No significant cross-reactivity or interference between CA9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CA9 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CA9 elisa kit
Intended Uses: This CA9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CA9. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CA9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CA9 antibody and an CA9-HRP conjugate. The assay sample and buffer are incubated together with CA9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA9 concentration since CA9 from samples and CA9-HRP conjugate compete for the anti-CA9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA9 from the sample, fewer sites are left to bind CA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA9 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CA9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CA9 antibody and an CA9-HRP conjugate. The assay sample and buffer are incubated together with CA9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA9 concentration since CA9 from samples and CA9-HRP conjugate compete for the anti-CA9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA9 from the sample, fewer sites are left to bind CA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA9 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CA9 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,698 Da
NCBI Official Full Name
carbonic anhydrase 9
NCBI Official Synonym Full Names
carbonic anhydrase IX
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250
UniProt Entry Name
CAH9_HUMAN
