Mouse Heat shock protein 90-alpha ELISA Kit | HSP90-alpha elisa kit

Mouse Heat shock protein 90-alpha ELISA Kit

Cat. Num. AAA27216

• Gene Names: HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
• Reactivity: Mouse
• Synonyms: Heat shock protein 90-alpha; N/A; Mouse Heat shock protein 90-alpha ELISA Kit; HSP90-alpha elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSP-90alpha. No significant cross-reactivity or interference between HSP-90alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP-90alpha and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for HSP90-alpha elisa kit

Principle of the Assay: HSP-90alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HSP-90alpha antibody and an HSP-90alpha-HRP conjugate. The assay sample and buffer are incubated together with HSP-90alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP-90alpha concentration since HSP-90alpha from samples and HSP-90alpha-HRP conjugate compete for the anti-HSP-90alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP-90alpha from the sample, fewer sites are left to bind HSP-90alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-90alpha concentration in each sample is interpolated from this standard curve.

Intended Uses: This HSP-90alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HSP-90alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for HSP90-alpha elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 154146191
• NCBI GeneID: 3320
• NCBI Accession #: NP_005339.3
• NCBI GenBank Nucleotide #: NM_005348.3
• Molecular Weight: 98,161 Da
• NCBI Official Full Name: heat shock protein HSP 90-alpha isoform 2
• NCBI Official Synonym Full Names: heat shock protein 90kDa alpha (cytosolic), class A member 1
• NCBI Official Symbol: HSP90AA1
• NCBI Official Synonym Symbols: EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
• NCBI Protein Information: heat shock protein HSP 90-alpha; HSP 86; heat shock 86 kDa; LPS-associated protein 2; heat shock 90kD protein 1, alpha; heat shock 90kDa protein 1, alpha; renal carcinoma antigen NY-REN-38; heat shock 90kD protein, alpha-like 4; epididymis luminal secretory protein 52; heat shock 90kD protein 1, alpha-like 4; lipopolysaccharide-associated protein 2
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• UniProt Gene Name: HSP90AA1
• UniProt Synonym Gene Names: HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2
• UniProt Entry Name: HS90A_HUMAN