Human Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase (GNE) ELISA Kit | GNE elisa kit

Human Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase (GNE) ELISA Kit

Cat. Num. AAA27319

• Gene Names: GNE; NM; DMRV; IBM2; Uae1; GLCNE
• Reactivity: Human
• Synonyms: Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase (GNE); N/A; Human Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase (GNE) ELISA Kit; GNE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GNE. No significant cross-reactivity or interference between GNE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GNE and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GNE elisa kit

Principle of the Assay: GNE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GNE antibody and an GNE-HRP conjugate. The assay sample and buffer are incubated together with GNE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNE concentration since GNE from samples and GNE-HRP conjugate compete for the anti-GNE antibody binding site. Since the number of sites is limited, as more sites are occupied by GNE from the sample, fewer sites are left to bind GNE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNE concentration in each sample is interpolated from this standard curve.

Intended Uses: This GNE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GNE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GNE elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4885285
• NCBI GeneID: 10020
• NCBI Accession #: NP_005467.1
• NCBI GenBank Nucleotide #: NM_005476.5
• Molecular Weight: 66,784 Da
• NCBI Official Full Name: bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 2
• NCBI Official Synonym Full Names: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• NCBI Official Symbol: GNE
• NCBI Official Synonym Symbols: NM; DMRV; IBM2; Uae1; GLCNE
• NCBI Protein Information: bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; N-acylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
• UniProt Protein Name: Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
• UniProt Gene Name: GNE
• UniProt Synonym Gene Names: GLCNE
• UniProt Entry Name: GLCNE_HUMAN