Rabbit N Cadherin Monoclonal Antibody | anti-NCAD antibody

N Cadherin Antibody

Cat. Num. AAA30054

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: N Cadherin, Antibody; N Cadherin Antibody; CADH2_HUMAN antibody; Cadherin 2 antibody; Cadherin 2 N cadherin neuronal antibody; Cadherin 2 type 1 antibody; Cadherin 2 type 1 N cadherin neuronal antibody; Cadherin 2; type 1; N-cadherin (neuronal) antibody; Cadherin-2 antibody; Cadherin2 antibody; Calcium dependent adhesion protein neuronal antibody; CD325 antibody; CD325 antigen antibody; CDH2 antibody; CDHN antibody; CDw325 antibody; CDw325 antigen antibody; N cadherin 1 antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; OTTHUMP00000066304 antibody; OTTHUMP00000067378 antibody; anti-NCAD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: SY02-46
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-NCAD antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-5000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with N Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining N Cadherin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining N Cadherin in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining N Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining N Cadherin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of N Cadherin on different lysates using anti-N Cadherin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: Mouse brain)

Related Product Information for anti-NCAD antibody

Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.

Product Categories/Family for anti-NCAD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14589889
• NCBI GeneID: 1000
• NCBI Accession #: NP_001783.2
• NCBI GenBank Nucleotide #: NM_001792.3
• UniProt Accession #: P19022; Q14923; Q8N173; B0YIY6
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin
• UniProt Entry Name: CADH2_HUMAN