Human Proteasome subunit beta type-4 (PSMB4) ELISA Kit | PSMB4 elisa kit

Human Proteasome subunit beta type-4 (PSMB4) ELISA Kit

Cat. Num. AAA27399

• Gene Names: PSMB4; HN3; HsN3; PROS26; PROS-26
• Reactivity: Human
• Synonyms: Proteasome subunit beta type-4 (PSMB4); N/A; Human Proteasome subunit beta type-4 (PSMB4) ELISA Kit; PSMB4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSMbeta4. No significant cross-reactivity or interference between PSMbeta4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSMbeta4 and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 264
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

Standard Curve (Sample)

Related Product Information for PSMB4 elisa kit

Principle of the Assay: PSMbeta4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PSMbeta4 antibody and an PSMbeta4-HRP conjugate. The assay sample and buffer are incubated together with PSMbeta4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSMbeta4 concentration since PSMbeta4 from samples and PSMbeta4-HRP conjugate compete for the anti-PSMbeta4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PSMbeta4 from the sample, fewer sites are left to bind PSMbeta4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSMbeta4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This PSMbeta4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PSMbeta4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 22538467
• NCBI GeneID: 5692
• NCBI Accession #: NP_002787.2
• NCBI GenBank Nucleotide #: NM_002796.2
• UniProt Accession #: P28070; P31148; Q5SZS5; Q6IBI4; Q969L6; B2R9L3
• Molecular Weight: 29,204 Da
• NCBI Official Full Name: proteasome subunit beta type-4
• NCBI Official Synonym Full Names: proteasome subunit beta 4
• NCBI Official Symbol: PSMB4
• NCBI Official Synonym Symbols: HN3; HsN3; PROS26; PROS-26
• NCBI Protein Information: proteasome subunit beta type-4
• UniProt Protein Name: Proteasome subunit beta type-4
• UniProt Gene Name: PSMB4
• UniProt Synonym Gene Names: PROS26; HsBPROS26; PROS-26; HsN3