Human Anti-M3 AChR (Anti-M3 Acetylcholine Receptor Antibody) ELISA Kit | Anti-M3 AChR elisa kit
Human Anti-M3 AChR (Anti-M3 Acetylcholine Receptor Antibody) ELISA Kit
Reactivity
Human
Synonyms
Anti-M3 AChR (Anti-M3 Acetylcholine Receptor Antibody); N/A; Human Anti-M3 AChR (Anti-M3 Acetylcholine Receptor Antibody) ELISA Kit; Anti-M3 AChR elisa kit
Reactivity
Human
Specificity
Specifically recognize Anti-M3 AChR, no obvious cross reaction with other analogues
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
3.125-200ng/ml
Sensitivity
1.875ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for Anti-M3 AChR elisa kit
Background: Anti-M3 Acetylcholine Receptor Antibody could be not only potential pathologic auto-antibodies, but also new diagnostic makers and therapeutic targets for SS. Sj gren's syndrome (SS) is an autoimmune disease that affects exocrine glands including salivary and lacrimal glands.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto the 96-well plate. The biotin conjugated antigen was used as the detection antigen. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antigen was added to bind with Anti-M3 AChR conjugated on coated antigen. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of target antigen in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto the 96-well plate. The biotin conjugated antigen was used as the detection antigen. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antigen was added to bind with Anti-M3 AChR conjugated on coated antigen. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of target antigen in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.
