General Spermine ELISA Kit
Spermine ELISA Kit
Reactivity
General
Synonyms
Spermine; N/A; Spermine ELISA Kit; Spermine elisa kit
Reactivity
General
Specificity
Specifically recognize Spermine, no obvious cross reaction with other analogues
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
12.5-800ng/ml
Sensitivity
7.5ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for Spermine elisa kit
Background: Spermine is an endogenous polyamine bearing multiple amino groups. It has been found to play important roles in cellular metabolism in all eukaryotic cells. It also can condense DNA in sperm due to its positive charge in the physiological condition. Because spermine is an endogenous molecule widely present in the biological environment, it is expected that spermine will have better biocompatibility then other synthetic cationic molecules.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Spermine. During the reaction, Spermine in the sample or standard competes with a fixed amount of Spermine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Spermine. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Spermine in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Spermine. During the reaction, Spermine in the sample or standard competes with a fixed amount of Spermine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Spermine. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Spermine in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
