Rabbit anti-Human, Mouse MEK3/MKK3 Polyclonal Antibody | anti-MAP2K3 antibody

Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal

Cat. Num. AAA27720

• Gene Names: MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MEK3/MKK3, Antibody; Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal; MAP2K3/MKK3 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase kinase 3; Anti-MAPKK3 Antibody; Anti-MEK3 Antibody; Anti-MKK3 Antibody; Anti-PRKMK3 Antibody; Anti-SAPKK-2 Antibody; Anti-SAPKK2 Antibody; anti-MAP2K3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MEK3/MKK3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MAP2K3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-5uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human MAP2K3/MKK3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MAP2K3/MKK3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(MAP2K3 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg HeLa Whole Cell Lysate 2 uL anti-MAP2K3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MAP2K3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :39 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of MAP2K3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MAP2K3 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)

IHC (Immunohistochemistry)

(Immunochemical staining MAP2K3 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining MAP2K3 in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining MAP2K3 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining MAP2K3 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-MAP2K3 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:39 kDa Observed band size:39 kDa)

Related Product Information for anti-MAP2K3 antibody

MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3, also known as MKK3) is a Protein Coding gene. The protein encoded by this gene is a dual-specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress and participates in the MAP kinase-mediated signaling cascade. 3 alternatively spliced human isoforms have been reported. MAP2K3 is widely expressed in bone marrow, gall bladder, and other tissues. MAP2K3 resides in a biological pathway linked to memory. It is in a signaling cascade associated with beta-amyloid mediated apoptosis and has enriched expression in microglia.MAP2K3 may represent a novel therapeutic target for age-related memory decline and perhaps Alzheimer's disease (AD).

Product Categories/Family for anti-MAP2K3 antibody

Death Receptor Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 21618347
• NCBI GeneID: 5606
• NCBI Accession #: NP_002747.2
• NCBI GenBank Nucleotide #: NM_002756.4
• UniProt Accession #: P46734; Q99441; Q9UE71; Q9UE72; B3KSK7
• Molecular Weight: 39,318 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 3 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 3
• NCBI Official Symbol: MAP2K3
• NCBI Official Synonym Symbols: MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 3; MEK 3; MAPKK 3; SAPK kinase 2; MAPK/ERK kinase 3; MAP kinase kinase 3; stress-activated protein kinase kinase 2
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• UniProt Gene Name: MAP2K3
• UniProt Synonym Gene Names: MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2
• UniProt Entry Name: MP2K3_HUMAN