Mouse Sialic acid ELISA Kit | SA elisa kit
Mouse Sialic acid ELISA Kit
Reactivity
Mouse
Synonyms
Sialic acid; N/A; Mouse Sialic acid ELISA Kit; SA elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 mg/L.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for SA elisa kit
Intended Uses: This SA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SA. This ELISA kit for research use only!
Principle of the Assay: SA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SA antibody and an SA-HRP conjugate. The assay sample and buffer are incubated together with SA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SA concentration since SA from samples and SA-HRP conjugate compete for the anti-SA antibody binding site. Since the number of sites is limited, as more sites are occupied by SA from the sample, fewer sites are left to bind SA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SA concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SA antibody and an SA-HRP conjugate. The assay sample and buffer are incubated together with SA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SA concentration since SA from samples and SA-HRP conjugate compete for the anti-SA antibody binding site. Since the number of sites is limited, as more sites are occupied by SA from the sample, fewer sites are left to bind SA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SA concentration in each sample is interpolated from this standard curve.
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