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IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)
Rabbit anti-Human EpCAM Monoclonal Antibody | anti-EpCAM antibody
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Western Blot, ELISA, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Synonyms
EpCAM; Monoclonal Antibody; Recombinant Anti-EpCAM Antibody; Rabbit Monoclonal; EpCAM/TROP-1/TACSTD1 Antibody; Rabbit MAb; epithelial cell adhesion molecule; Anti-DIAR5 Antibody; Anti-EGP-2 Antibody; Anti-EGP314 Antibody; Anti-EGP40 Antibody; Anti-ESA Antibody; Anti-HNPCC8 Antibody; Anti-KS1/4 Antibody; Anti-KSA Antibody; Anti-M4S1 Antibody; Anti-MIC18 Antibody; Anti-MK-1 Antibody; Anti-TACSTD1 Antibody; Anti-TROP-1 Antibody; Anti-TROP1 Antibody; anti-EpCAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
28
Specificity
Human EpCAM/TROP1/CD326.
No cross-reactivity in ELISA with Human CD146/MCAM, Human CD171/N-CAML1/L1CAM, Human OBCAM/OPCML, Human TROP2/TACSTD2
No cross-reactivity in ELISA with Human CD146/MCAM, Human CD171/N-CAML1/L1CAM, Human OBCAM/OPCML, Human TROP2/TACSTD2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EpCAM antibody
Western Blot (WB), ELISA (EIA), ELISA (EIA) (Det), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:1000-1:5000
ICC/IF: 1:250-1:5000
FC/FACS/FCM: 1:100-1:500
IP: 0.2-1uL/mg of lysate
ELISA (Det): 1:5000-1:50,000
ELISA (Det): This antibody will detect Human EpCAM/TROP-1/TACSTD1 in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
ELISA: 1:25,000-1:50,000
IHC-P: 1:1000-1:5000
ICC/IF: 1:250-1:5000
FC/FACS/FCM: 1:100-1:500
IP: 0.2-1uL/mg of lysate
ELISA (Det): 1:5000-1:50,000
ELISA (Det): This antibody will detect Human EpCAM/TROP-1/TACSTD1 in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human EpCAM protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM/TROP1 (rh EpCAM).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)
WB (Western Blot)
(Anti-EpCAM rabbit monoclonal antibody at 1:500 dilutionLane A: MCF-7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:38 kDa)
WB (Western Blot)
(Anti-EpCAM rabbit monoclonal antibody at 1:500 dilutionLane A: MCF-7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:38 kDa)
FCM (Flow Cytometry)
(Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.)
FCM (Flow Cytometry)
(Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.)
IHC (Immunohistochemistry)
(Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.)
IHC (Immunohistochemistry)
(Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.)
IHC (Immunohistochemistry)
(Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium.)
IHC (Immunohistochemistry)
(Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.)
Related Product Information for anti-EpCAM antibody
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
References
Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28.2kDa (248aa) 28-40kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
tumor-associated calcium signal transducer 1
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
UniProt Entry Name
EPCAM_HUMAN
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Product Notes
The EpCAM epcam (Catalog #AAA27736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EpCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), ELISA (EIA) (Det), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:1000-1:5000 ICC/IF: 1:250-1:5000 FC/FACS/FCM: 1:100-1:500 IP: 0.2-1uL/mg of lysate ELISA (Det): 1:5000-1:50,000 ELISA (Det): This antibody will detect Human EpCAM/TROP-1/TACSTD1 in ELISA pair set. In a sandwich ELISA, it can be used as detection antibody when paired with. Researchers should empirically determine the suitability of the EpCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EpCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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