Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us
Cart Login
product-image-AAA27756_IP6.png
IP (Immunoprecipitation)
>
<
product-image-AAA27756_IF5.png
product-image-AAA27756_IHC4.png
product-image-AAA27756_IHC3.png
product-image-AAA27756_IHC2.png
product-image-AAA27756_WB.png

Rabbit anti-Human MYEF2 Polyclonal Antibody | anti-MYEF2 antibody

Anti-MYEF2 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
MYEF2, Antibody; Anti-MYEF2 Antibody, Rabbit Polyclonal; MYEF2 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-HsT18564 Antibody; Anti-MST156 Antibody; Anti-MSTP156 Antibody; anti-MYEF2 antibody
Ordering
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
 
When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures.
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MYEF2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-MYEF2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:1000-1:20,000
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of Lysate
Immunogen
E Coli-derived Human MYEF2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MYEF2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IP (Immunoprecipitation)

(MYEF2 was immunoprecipitated using: Lane A:0.5 mg SH-SY5Y Whole Cell Lysate 4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 64 kDa Observed band size :64 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of MYEF2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MYEF2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry)

(Immunochemical staining of human MYEF2 in human kidney with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining of human MYEF2 in human brain with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining of human MYEF2 in human testis with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-MYEF2 rabbit polyclonal antibody at 1:500 dilution Lane A: SH-SY5Y Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:64 kDa Observed band size:70 kDa)

Similar Products

product-image-AAA27756_IP6.png

MYEF2 Antibody

Host: Rabbit
Reactivity: Human
Apps: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
product-image-AAA20930_WB4.jpg

VEGFR2 Antibody

Host: Rabbit
Reactivity: Sus scrofa; Porcine (Pig)
Apps: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
product-image-AAA16218_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA17008_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Bovine
Apps: n/a
product-image-AAA17023_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Porcine
Apps: n/a
product-image-AAA16833_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Canine
Apps: n/a
product-image-AAA16847_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Sheep
Apps: n/a
product-image-AAA22610_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Canine
Apps: n/a
product-image-AAA16761_SC.jpg

VEGF165 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA15241_SC.png

VEGFC ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA12621_SC.jpg

sVEGFR-2 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA12818_SC.jpg

sVEGFR-2 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA23328_SC.jpg

VEGFB ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a
product-image-AAA17717_SC.jpg

VEGF ELISA Kit

Host: n/a
Reactivity: Rabbit
Apps: n/a
product-image-AAA17213_SC.jpg

VEGF-A ELISA Kit

Host: n/a
Reactivity: Monkey
Apps: n/a
product-image-AAA15479_SC.png

VEGFA ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a
product-image-AAA16604_SC.jpg

VEGF.C ELISA Kit

Host: n/a
Reactivity: Rabbit
Apps: n/a
product-image-AAA15591_SC.png

VEGFB ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a
product-image-AAA23341_SC.png

VEGFA ELISA Kit

Host: n/a
Reactivity: Bovine
Apps: n/a
product-image-AAA15558_SC.png

VEGFA ELISA Kit

Host: n/a
Reactivity: Pig
Apps: n/a

Product Notes

The MYEF2 (Catalog #AAA27756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MYEF2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYEF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:1000-1:20,000 ICC/IF: 1:100-1:500 IP: 1-5uL/mg of Lysate. Researchers should empirically determine the suitability of the MYEF2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYEF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.