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IF (Immunofluorescence)
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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

Rabbit TFEB Polyclonal Antibody | anti-TFEB antibody

TFEB Polyclonal Antibody

Gene Names
TFEB; TCFEB; BHLHE35; ALPHATFEB
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity Purification
Synonyms
TFEB, Antibody; TFEB Polyclonal Antibody; TFEB; ALPHATFEB; BHLHE35; TCFEB; anti-TFEB antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Sequence Length
476
Applicable Applications for anti-TFEB antibody
Western Blot (WB), Immunohistochemistry (IHC-p), Immnofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IF/ICC 1:50 - 1:200
ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-173 of human TFEB (NP_009093.1)
Species/Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20 degrees C. Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of PC-12 cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of TFEB in paraffin-embedded rat testis tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of TFEB in paraffin-embedded rat testis tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of TFEB in paraffin-embedded mouse lung tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of TFEB in paraffin-embedded mouse lung tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of TFEB in paraffin-embedded human colon tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of TFEB in paraffin-embedded human colon tissue using TFEB Rabbit pAb (AAA28118) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) HeLa cells using [KD Validated] TFEB Rabbit pAb (AAA28118) at 1:1000 dilution incubated overnight at 4 degrees C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) HeLa cells using [KD Validated] TFEB Rabbit pAb (AAA28118) at 1:1000 dilution incubated overnight at 4 degrees C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

WB (Western Blot)

(Western blot analysis of various lysates, using TFEB Rabbit pAb (AAA28118) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of various lysates, using TFEB Rabbit pAb (AAA28118) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
Related Product Information for anti-TFEB antibody
Background: Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular response to amino acid starvation; lysosome localization; and positive regulation of autophagy. Located in cytosol; lysosomal membrane; and nucleoplasm.
Product Categories/Family for anti-TFEB antibody
References

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 65-70kDa
Calculated MW: 53kDa
NCBI Official Full Name
Transcription factor EB
NCBI Official Synonym Full Names
transcription factor EB
NCBI Official Symbol
TFEB
NCBI Official Synonym Symbols
TCFEB; BHLHE35; ALPHATFEB
NCBI Protein Information
transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
UniProt Protein Name
Transcription factor EB
UniProt Gene Name
TFEB
UniProt Synonym Gene Names
BHLHE35; bHLHe35
UniProt Entry Name
TFEB_HUMAN

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Product Notes

The TFEB tfeb (Catalog #AAA28118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TFEB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-p), Immnofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA: Recommended starting concentration is 1 ug/mL. Please optimize the concentration based on your specific assay requirements. Researchers should empirically determine the suitability of the TFEB tfeb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFEB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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