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IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUVBL1 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human, Mouse RUVBL1 Polyclonal Antibody | anti-RUVBL1 antibody
RUVBL1 Polyclonal Antibody
Gene Names
RUVBL1; RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
RUVBL1; Polyclonal Antibody; RUVBL1 Polyclonal Antibody; RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52; anti-RUVBL1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
456
Applicable Applications for anti-RUVBL1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human RUVBL1
Calculated Molecular Weight
50kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUVBL1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUVBL1 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RUVBL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RUVBL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Product Categories/Family for anti-RUVBL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
456
NCBI Official Full Name
RuvB-like 1
NCBI Official Synonym Full Names
RuvB-like AAA ATPase 1
NCBI Official Symbol
RUVBL1
NCBI Official Synonym Symbols
RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
NCBI Protein Information
ruvB-like 1; ECP-54; NMP 238; pontin 52; TAP54-alpha; INO80 complex subunit H; nuclear matrix protein 238; RuvB (E coli homolog)-like 1; 49 kDa TBP-interacting protein; TIP60-associated protein 54-alpha; 54 kDa erythrocyte cytosolic protein; TATA binding protein interacting protein 49 kDa; 49 kDa TATA box-binding protein-interacting protein
UniProt Protein Name
RuvB-like 1
UniProt Gene Name
RUVBL1
UniProt Synonym Gene Names
INO80H; NMP238; TIP49; TIP49A; 49 kDa TBP-interacting protein; ECP-54; NMP 238; TAP54-alpha
UniProt Entry Name
RUVB1_HUMAN
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Product Notes
The RUVBL1 ruvbl1 (Catalog #AAA28185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUVBL1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RUVBL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the RUVBL1 ruvbl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUVBL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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