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IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit MSH6 Polyclonal Antibody | anti-MSH6 antibody
MSH6 Rabbit pAb
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat, MK
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Synonyms
MSH6; Polyclonal Antibody; MSH6 Rabbit pAb; GTBP; GTMBP; HNPCC5; HSAP; p160; mutS homolog 6; anti-MSH6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, MK
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Sequence
KQRARELGLGRHENAIKYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWNNVDPYERDQLRVKME
Applicable Applications for anti-MSH6 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF/ICC: 1:50-1:200
IHC: 1:50-1:100
IF/ICC: 1:50-1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSH6 (NP_000170.1).
Cellular Location
Chromosome, Nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A-549 cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A-549 cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using MSH6 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using MSH6 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using MSH6 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using MSH6 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using MSH6 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using MSH6 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MSH6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MSH6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
Related Product Information for anti-MSH6 antibody
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.
Product Categories/Family for anti-MSH6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
81,890 Da
NCBI Official Full Name
calpain 1, (mu/I) large subunit, isoform CRA_c
NCBI Official Synonym Full Names
calpain 1, (mu/I) large subunit
NCBI Official Symbol
CAPN1
NCBI Official Synonym Symbols
CANP; muCL; CANP1; CANPL1; muCANP
NCBI Protein Information
calpain-1 catalytic subunit; CANP 1; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calpain, large polypeptide L1; calcium-activated neutral proteinase 1; cell proliferation-inducing protein 30; cell proliferation-inducing gene 30 protein
UniProt Protein Name
Calpain-1 catalytic subunit
UniProt Gene Name
CAPN1
UniProt Synonym Gene Names
CANPL1; CANP 1; muCANP
UniProt Entry Name
CAN1_HUMAN
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Product Notes
The MSH6 capn1 (Catalog #AAA28426) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MSH6 Rabbit pAb reacts with Human, Mouse, Rat, MK and may cross-react with other species as described in the data sheet. AAA Biotech's MSH6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IHC: 1:50-1:100 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the MSH6 capn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KQRARELGLG RHENAIKYLG QDYEQLRVRC LQSGTLFRDE AFPPVPQSLG YKDLGPNSSK TYGIKWKRPT ELLSNPQFIV DGATRTDICQ GALGDCWLLA AIASLTLNDT LLHRVVPHGQ SFQNGYAGIF HFQLWQFGEW VDVVVDDLLP IKDGKLVFVH SAEGNEFWSA LLEKAYAKVN GSYEALSGGS TSEGFEDFTG GVTEWYELRK APSDLYQIIL KALERGSLLG CSIDISSVLD MEAITFKKLV KGHAYSVTGA KQVNYRGQVV SLIRMRNPWG EVEWTGAWSD SSSEWNNVDP YERDQLRVKM E. It is sometimes possible for the material contained within the vial of "MSH6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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