WB (Western Blot)
(Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Mouse RPS6 Monoclonal Antibody | anti-RPS6 antibody
RPS6 Antibody (N-term)
IF~~1:25
IHC~~1:25
WB~~1:2000
WB (Western Blot)
(Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
Heinze H.,et al.J. Biol. Chem. 263:4139-4144(1988).
Antoine M.,et al.Hum. Mol. Genet. 1:565-570(1992).
Pata I.,et al.Gene 121:387-392(1992).
Shichijo S.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
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Product Notes
The RPS6 rps6 (Catalog #AAA28643) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RPS6 Antibody (N-term) reacts with Human, rat (Predicted Reactivity: Monkey, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF). FC~~1:25 IF~~1:25 IHC~~1:25 WB~~1:2000. Researchers should empirically determine the suitability of the RPS6 rps6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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