Rabbit anti-Human CD9 Polyclonal Antibody | anti-CD9 antibody

CD9 Antibody (Center)

Cat. Num. AAA28656

• Gene Names: CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CD9, Antibody; CD9 Antibody (Center); CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD9; MIC3; TSPAN29; anti-CD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the Central region of human CD9.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.27 (varies by lot)
• Sequence Positions: 115-145
• Sequence Length: 228

• Applicable Applications for anti-CD9 antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of lysate from Hela cell line, using CD9 Antibody (Center). AAA28656 was diluted at 1:1000. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

WB (Western Blot)

(Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center). AAA28656 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot)

(Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center). AAA28656 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot)

(Western blot analysis of lysate from mouse kidney tissue lysate, using CD9 Antibody (Center). AAA28656 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

WB (Western Blot)

(Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center). AAA28656 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot)

(Anti-CD9 Antibody (Center)at 1:2000 dilution + MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CD9 antibody

CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.

Product Categories/Family for anti-CD9 antibody

Cardiovascular; Developmental Biology; Immunology; Signal Transduction

References

Ovalle,S., Int. J. Cancer 121 (10), 2140-2152 (2007)
Kovalenko,O.V., Mol. Cell Proteomics 6 (11), 1855-1867 (2007)
Abache,T., J. Cell. Biochem. 102 (3), 650-664 (2007)
Horejsi,V., FEBS Lett. 288 (1-2), 1-4 (1991)

NCBI and Uniprot Product Information

• NCBI GI #: 4502693
• NCBI GeneID: 928
• NCBI Accession #: NP_001760.1
• NCBI GenBank Nucleotide #: NM_001769.3
• UniProt Accession #: P21926; Q5J7W6; Q96ES4; D3DUQ9
• Molecular Weight: 25416
• NCBI Official Full Name: CD9 antigen
• NCBI Official Synonym Full Names: CD9 molecule
• NCBI Official Symbol: CD9
• NCBI Official Synonym Symbols: MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• NCBI Protein Information: CD9 antigen
• UniProt Protein Name: CD9 antigen
• UniProt Gene Name: CD9
• UniProt Synonym Gene Names: MIC3; TSPAN29; MRP-1; Tspan-29
• UniProt Entry Name: CD9_HUMAN