Rabbit anti-Rat, Cow OTUD1 Polyclonal Antibody | anti-OTUD1 antibody

OTUD1 Polyclonal Antibody

Cat. Num. AAA28819

• Gene Names: OTUD1; DUBA7; OTDC1
• Reactivity: Rat, Cow
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: OTUD1, Antibody; OTUD1 Polyclonal Antibody; OTU domain-containing protein 1; 3.4.19.12; DUBA-7; OTUD1; DUBA7; OTDC1; anti-OTUD1 antibody

• Host: Rabbit
• Reactivity: Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)

• Applicable Applications for anti-OTUD1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistchemistry-Paraffin)

(Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

IHC (Immunohistochemistry)

(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

WB (Western Blot)

(Sample: Testis (Mouse)Lysate at 40 ug Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 60kD)

WB (Western Blot)

(Dilution: Sample: HepG2 (Human) Cell Lysate at 40 ug Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 60kD)

WB (Western Blot)

(Sample: 293T (Human)Cell Lysate at 40 ug Primary: Anti-OTUD1(bs-17563R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 60kD)

IHC (Immunohistochemistry-Paraffin)

(Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 223941923
• NCBI GeneID: 220213
• NCBI Accession #: NP_001138845.1
• NCBI GenBank Nucleotide #: NM_001145373.2
• UniProt Accession #: Q5VV17
• Molecular Weight: 51,063 Da
• NCBI Official Full Name: OTU domain-containing protein 1
• NCBI Official Synonym Full Names: OTU deubiquitinase 1
• NCBI Official Symbol: OTUD1
• NCBI Official Synonym Symbols: DUBA7; OTDC1
• NCBI Protein Information: OTU domain-containing protein 1; DUBA-7; OTU domain containing 1
• UniProt Protein Name: OTU domain-containing protein 1
• UniProt Gene Name: OTUD1
• UniProt Synonym Gene Names: DUBA7; OTDC1
• UniProt Entry Name: OTUD1_HUMAN