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FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse SIRT3 Polyclonal Antibody | anti-SIRT3 antibody
SIRT3 Antibody
Gene Names
SIRT3; SIR2L3
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified
Synonyms
SIRT3; Polyclonal Antibody; SIRT3 Antibody; hSIRT 3 antibody; hSIRT3 antibody; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody; NAD dependent deacetylase sirtuin 3 mitochondrial antibody; NAD-dependent protein deacetylase sirtuin-3; mitochondrial antibody; Regulatory protein SIR2 homolog 3 antibody; Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody; Sir 2 like 3 antibody; SIR 2 like protein 3 antibody; SIR 3 antibody; SIR2 L3 antibody; Sir2 like 3 antibody; SIR2 like protein 3 antibody; SIR2-like protein 3 antibody; SIR2L3 antibody; SIR3_HUMAN antibody; SIRT 3 antibody; SIRT3 antibody; Sirtuin 3 antibody; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody; Sirtuin type 3 antibody; Sirtuin3 antibody; anti-SIRT3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SIRT3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of HepG2 cells using anti-SIRT3 rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of HepG2 cells using anti-SIRT3 rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of MCF-7 cells using anti-SIRT3 rabbit polyclonal antibody.)
ICC (Immunocytochemistry)
(Immunocytochemical staining of MCF-7 cells using anti-SIRT3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-SIRT3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-SIRT3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-SIRT3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-SIRT3 rabbit polyclonal antibody.)
WB (Western Blot)
(Western blot analysis on different lysates using anti-SIRT3 rabbit polyclonal antibody. Positive control: Lane 1: A172 Lane 2: Mouse liver Lane 3: NIH/3T3 Lane 4: Mouse kidney Lane 5: F9)
WB (Western Blot)
(Western blot analysis on different lysates using anti-SIRT3 rabbit polyclonal antibody. Positive control: Lane 1: A172 Lane 2: Mouse liver Lane 3: NIH/3T3 Lane 4: Mouse kidney Lane 5: F9)
Related Product Information for anti-SIRT3 antibody
The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.
Product Categories/Family for anti-SIRT3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,573 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b
NCBI Official Synonym Full Names
sirtuin 3
NCBI Official Symbol
SIRT3
NCBI Official Synonym Symbols
SIR2L3
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae,
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial
UniProt Gene Name
SIRT3
UniProt Synonym Gene Names
SIR2L3; hSIRT3
UniProt Entry Name
SIR3_HUMAN
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Product Notes
The SIRT3 sirt3 (Catalog #AAA29877) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SIRT3 sirt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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