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FCM (Flow Cytometry)
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Mouse anti-Human EIF2A Monoclonal Antibody | anti-EIF2A antibody

EIF2A Antibody

Gene Names
EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
EIF2A, Antibody; EIF2A Antibody; 65 kDa eukaryotic translation initiation factor 2A antibody; CDA 02 antibody; CDA02 antibody; EIF 2 antibody; EIF 2A antibody; eIF-2A antibody; EIF2 antibody; eif2a antibody; EIF2A_HUMAN antibody; Eukaryotic translation initiation factor 2A 65kDa antibody; Eukaryotic translation initiation factor 2A antibody; MST089 antibody; MSTP004 antibody; MSTP089 antibody; anti-EIF2A antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
A8-A7
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-EIF2A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

ICC (Immunocytochemistry)

(ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1, 000 dilution. Positive control�� Line1: MCF-7 Line2: PC-12 Line3: HepG2 Line4: Hela Line5: Cos7 Line6: K562 Line7: Jurkat Line8: A431 Line9:)

WB (Western Blot)

(Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1, 000 dilution.)

WB (Western Blot)

(Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1, 000 dilution.)

Related Product Information for anti-EIF2A antibody
eIF2A (eukaryotic translation initiation factor 2A), also known as CDA02, MSTP004 or MSTP089, is a 585 amino acid protein that contains three WD repeats. Expressed ubiquitously with highest expression in heart, brain, pancreas and placenta, eIF2A functions as a translation initiation factor that binds Met-tRNA and directs it to 40S ribosomal subunits. Present in the early steps of protein synthesis, eIF2A controls the binding of Met-tRNA to 40S ribosomal subunits in a codon-dependent manner, in contrast to the eIF2 complex which accomplishes the same task in a GTP-dependent manner. In addition to its role in transcription initiation, eIF2A may also act to negatively regulate the expression of specific proteins, suggesting a possible role as a transcriptional repressor. eIF2A exists as two isoforms due to alternative splicing events.
Product Categories/Family for anti-EIF2A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,990 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2A, 65kDa
NCBI Official Symbol
EIF2A
NCBI Official Synonym Symbols
CDA02; EIF-2A; MST089; MSTP004; MSTP089
NCBI Protein Information
eukaryotic translation initiation factor 2A; 65 kDa eukaryotic translation initiation factor 2A
UniProt Protein Name
Eukaryotic translation initiation factor 2A
UniProt Gene Name
EIF2A
UniProt Synonym Gene Names
eIF-2A
UniProt Entry Name
EIF2A_HUMAN

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Product Notes

The EIF2A eif2a (Catalog #AAA29904) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF2A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the EIF2A eif2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.