Human N acetyl beta D glucosaminidase ELISA Kit | NAG elisa kit

Human N acetyl beta D glucosaminidase ELISA Kit

Cat. Num. AAA16242

• Reactivity: Human
• Synonyms: N acetyl beta D glucosaminidase; N/A; Human N acetyl beta D glucosaminidase ELISA Kit; Human N acetyl b D glucosaminidase ELISA Kit; NAG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NAG. No significant cross-reactivity or interference between NAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NAG and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for NAG elisa kit

Principle of the Assay: NAG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NAG. Standards or samples are then added to the microtiter plate wells and NAG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NAG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for NAG are added to each well to "sandwich" the NAG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NAG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAG concentration in each sample is interpolated from this standard curve.

Intended Uses: This NAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 2506027
• Molecular Weight: 137,535 Da
• NCBI Official Full Name: NAG, partial
• UniProt Protein Name: Bifunctional autolysin
• UniProt Gene Name: atl
• UniProt Synonym Gene Names: nag
• UniProt Entry Name: ATL_STAAU