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ICC (Immunocytochemistry)
(ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit MEK1/2 Monoclonal Antibody | anti-MEK1/2 antibody
MEK1/2 Antibody
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
MEK1/2; Monoclonal Antibody; MEK1/2 Antibody; AA589381 antibody; CFC3 antibody; Dual specificity mitogen-activated protein kinase kinase 1 antibody; Dual specificity mitogen-activated protein kinase kinase 2 antibody; EC 2.7.12.2 antibody; ERK activator kinase 1 antibody; ERK activator kinase 2 antibody; FLJ26075 antibody; MAP kinase kinase 1 antibody; MAP kinase kinase 2 antibody; MAP2K1 antibody; MAP2K2 antibody; MAPK/ERK kinase 1 antibody; MAPK/ERK kinase 2 antibody; MAPKK 1 antibody; MAPKK1 antibody; MAPKK2 antibody; MEK 1 antibody; MEK1 antibody; MEKK1 antibody; Mitogen activated protein kinase kinase 1 antibody; Mitogen activated protein kinase kinase 2 antibody; Mitogen-activated protein kinase kinase 2; p45 antibody; MK2 antibody; MKK 1 antibody; MKK 2 antibody; MKK1 antibody; MKK2 antibody; MP2K1_HUMAN antibody; PRKMK 1 antibody; PRKMK 2 antibody; Prkmk1 antibody; Prkmk2 antibody; protein kinase; mitogen-activated; kinase 1 (MAP kinase kinase 1) antibody; Protein kinase; kinase 1 antibody; kinase 2 antibody; anti-MEK1/2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
SR13-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-MEK1/2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: PC12 Lane 3: NIH/3T3)
WB (Western Blot)
(Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: PC12 Lane 3: NIH/3T3)
Related Product Information for anti-MEK1/2 antibody
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Product Categories/Family for anti-MEK1/2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
400
NCBI Official Full Name
Dual specificity mitogen-activated protein kinase kinase 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 2
NCBI Official Symbol
MAP2K2
NCBI Official Synonym Symbols
CFC4; MEK2; MKK2; MAPKK2; PRKMK2
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 2; MAPK/ERK kinase 2; MAP kinase kinase 2; ERK activator kinase 2; mitogen-activated protein kinase kinase 2, p45
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 2
UniProt Gene Name
MAP2K2
UniProt Synonym Gene Names
MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
UniProt Entry Name
MP2K2_HUMAN
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Product Notes
The MEK1/2 map2k2 (Catalog #AAA29992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MEK1/2 Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the MEK1/2 map2k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1/2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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