Rabbit Prohibitin Monoclonal Antibody | anti-PHB antibody

Prohibitin Antibody

Cat. Num. AAA29994

• Gene Names: PHB; PHB1
• Reactivity: Human, Mouse, Rat, Zebrafish
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: Prohibitin, Antibody; Prohibitin Antibody; Epididymis luminal protein 215 antibody; Epididymis secretory sperm binding protein Li 54e antibody; HEL 215 antibody; HEL S 54e antibody; PHB antibody; PHB_HUMAN antibody; PHB1 antibody; Prohibitin antibody; anti-PHB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Zebrafish
• Clonality: Monoclonal
• Clone Number: SR46-02
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-PHB antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:200-1:500; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.)

WB (Western Blot)

(Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2)

Related Product Information for anti-PHB antibody

Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed.

Product Categories/Family for anti-PHB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 246483
• NCBI GeneID: 5245
• UniProt Accession #: P35232; Q4VBQ0
• Molecular Weight: 29,804 Da
• NCBI Official Full Name: prohibitin
• NCBI Official Synonym Full Names: prohibitin
• NCBI Official Symbol: PHB
• NCBI Official Synonym Symbols: PHB1
• NCBI Protein Information: prohibitin
• UniProt Protein Name: Prohibitin
• UniProt Gene Name: PHB
• UniProt Entry Name: PHB_HUMAN