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FCM (Flow Cytometry)
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Rabbit SUMO4 Monoclonal Antibody | anti-SUMO4 antibody

SUMO4 Antibody

Gene Names
SUMO4; IDDM5; SMT3H4; SUMO-4; dJ281H8.4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
SUMO4, Antibody; SUMO4 Antibody; dJ281H8.4 antibody; IDDM5 antibody; Small ubiquitin like modifier 4 protein antibody; Small ubiquitin-like protein 4 antibody; Small ubiquitin-related modifier 4 antibody; SMT3 suppressor of mif two 3 homolog 2 antibody; SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae) antibody; SMT3H4 antibody; SUMO 4 antibody; SUMO-4 antibody; SUMO4 antibody; SUMO4_HUMAN antibody; anti-SUMO4 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JJ085-01
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-SUMO4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of 293 cells with SUMO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry)

(ICC staining SUMO4 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining SUMO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining SUMO4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining SUMO4 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot)

(Western blot analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: Jurkat)

Related Product Information for anti-SUMO4 antibody
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2, SUMO-3 and SUMO-4, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Ubiquitin and SUMO proteins utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2, SUMO-3 and SUMO-4 proteins localize to the nucleus. In contrast to the other SUMO proteins, SUMO-4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90, which may impair processing to mature form and conjugation to substrates. It is suggested that defects in the gene that encodes for the SUMO-4 protein may be involved in the pathogenesis of type I diabetes.
Product Categories/Family for anti-SUMO4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
95
NCBI Official Full Name
Small ubiquitin-related modifier 4
NCBI Official Synonym Full Names
small ubiquitin-like modifier 4
NCBI Official Symbol
SUMO4
NCBI Official Synonym Symbols
IDDM5; SMT3H4; SUMO-4; dJ281H8.4
NCBI Protein Information
small ubiquitin-related modifier 4; insulin-dependent diabetes mellitus 5; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 4; small ubiquitin-like modifier 4 protein
UniProt Protein Name
Small ubiquitin-related modifier 4
UniProt Gene Name
SUMO4
UniProt Synonym Gene Names
SMT3H4; SUMO-4
UniProt Entry Name
SUMO4_HUMAN

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Product Notes

The SUMO4 sumo4 (Catalog #AAA30248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUMO4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUMO4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SUMO4 sumo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUMO4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.