Human DNA topoisomerase 1 ELISA Kit | TOP1 elisa kit
Human DNA topoisomerase 1 ELISA Kit
Gene Names
TOP1; TOPI
Reactivity
Human
Synonyms
DNA topoisomerase 1; N/A; Human DNA topoisomerase 1 ELISA Kit; TOP1/DNA topoisomerase I; TOP1 elisa kit
Reactivity
Human
Specificity
Specifically recognize TOP1, no obvious cross reaction with other analogues
Sequence Length
765
Assay Type
Sandwich
Samples
Cell lysates, tissue homogenates and other biological samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for TOP1 elisa kit
Background: DNA topoisomerase 1 (TOP1), an essential manipulator of DNA topology during RNA transcription and DNA replication processes, can prevent R-loop accumulation by removing the positive and negative DNA supercoiling that is made by RNA polymerases during transcription. Diseases associated with TOP1 include Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Neutropenia. Among its related pathways are Transport of the SLBP independent Mature mRNA and Metabolism of proteins.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TOP1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TOP1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TOP1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TOP1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TOP1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TOP1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TOP1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TOP1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,726 Da
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
NCBI Protein Information
DNA topoisomerase 1
UniProt Protein Name
DNA topoisomerase 1
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN
