Standard Curve (Sample)
Human DNA topoisomerase 1 ELISA Kit | TOP1 elisa kit
Human DNA topoisomerase 1 ELISA Kit
Gene Names
TOP1; TOPI
Reactivity
Human
Synonyms
DNA topoisomerase 1; N/A; Human DNA topoisomerase 1 ELISA Kit; TOP1/DNA topoisomerase I; TOP1 elisa kit
Reactivity
Human
Specificity
Specifically recognize TOP1, no obvious cross reaction with other analogues
Sequence Length
765
Assay Type
Sandwich
Samples
Cell lysates, tissue homogenates and other biological samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TOP1 elisa kit
Background: DNA topoisomerase 1 (TOP1), an essential manipulator of DNA topology during RNA transcription and DNA replication processes, can prevent R-loop accumulation by removing the positive and negative DNA supercoiling that is made by RNA polymerases during transcription. Diseases associated with TOP1 include Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy 1 and Neutropenia. Among its related pathways are Transport of the SLBP independent Mature mRNA and Metabolism of proteins.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TOP1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TOP1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TOP1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TOP1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti TOP1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti TOP1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with TOP1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of TOP1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,726 Da
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
NCBI Protein Information
DNA topoisomerase 1
UniProt Protein Name
DNA topoisomerase 1
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN
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Product Notes
The Human TOP1 top1 (Catalog #AAA17592) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17592 ELISA Kit recognizes Human TOP1. It is sometimes possible for the material contained within the vial of "DNA topoisomerase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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