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FCM (Flow Cytometry)
(Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit PTP1B Monoclonal Antibody | anti-PTP1B antibody
PTP1B Antibody
Gene Names
PTPN1; PTP1B
Reactivity
Human, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PTP1B; Monoclonal Antibody; PTP1B Antibody; PTP1B antibody; Non receptor tyrosine phosphatase 1 antibody; Protein phosphotyrosylphosphatase 1B antibody; Protein tyrosine phosphatase 1B antibody; Protein tyrosine phosphatase non receptor type 1 antibody; Protein tyrosine phosphatase placental antibody; Protein-tyrosine phosphatase 1B antibody; PTN1_HUMAN antibody; PTP 1B antibody; PTP-1B antibody; PTPN 1 antibody; PTPN1 antibody; Tyrosine protein phosphatase non receptor type 1 antibody; Tyrosine-protein phosphatase non-receptor type 1 antibody; anti-PTP1B antibody
Host
Rabbit
Reactivity
Human, Zebrafish
Clonality
Monoclonal
Clone Number
JJ0935
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PTP1B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM (Flow Cytometry)
(Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry)
(ICC staining PTP1B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PTP1B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PTP1B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PTP1B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PTP1B in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PTP1B in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTP1B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTP1B antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: MCF-7 Lane 3: HepG2 Lane 4: A431)
WB (Western Blot)
(Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: MCF-7 Lane 3: HepG2 Lane 4: A431)
Related Product Information for anti-PTP1B antibody
The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
Product Categories/Family for anti-PTP1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,967 Da
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 1
NCBI Official Symbol
PTPN1
NCBI Official Synonym Symbols
PTP1B
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 1
UniProt Gene Name
PTPN1
UniProt Synonym Gene Names
PTP1B; PTP-1B
UniProt Entry Name
PTN1_HUMAN
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Product Notes
The PTP1B ptpn1 (Catalog #AAA30257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTP1B Antibody reacts with Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's PTP1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PTP1B ptpn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTP1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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