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FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit Proteasome 20S C2 Monoclonal Antibody | anti-PSMA1 antibody
Proteasome 20S C2 Antibody
Gene Names
PSMA1; NU; HC2; PROS30; HEL-S-275
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
Proteasome 20S C2; Monoclonal Antibody; Proteasome 20S C2 Antibody; 30 kDa prosomal protein antibody; HC 2 antibody; HC2 antibody; Macropain subunit C2 antibody; Macropain subunit nu antibody; MGC14542 antibody; MGC14575 antibody; MGC14751 antibody; MGC1667 antibody; MGC21459 antibody; MGC22853 antibody; MGC23915 antibody; Multicatalytic endopeptidase complex subunit C2 antibody; NU antibody; PROS 30 antibody; PROS-30 antibody; PROS30 antibody; Proteasome (prosome macropain) subunit alpha type 1 antibody; Proteasome alpha 1 subunit antibody; Proteasome component C2 antibody; Proteasome nu chain antibody; Proteasome subunit alpha type 1 antibody; Proteasome subunit alpha type I antibody; Proteasome subunit alpha type-1 antibody; Proteasome subunit nu antibody; Protein P30 33K antibody; PSA1_HUMAN antibody; PSC 2 antibody; PSC2 antibody; PSMA 1 antibody; psmA1 antibody; anti-PSMA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB89-30
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PSMA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M Lane 2: K562 Lane 3: Rat testis Lane 4: Mouse testis Lane 5: Mouse colon)
WB (Western Blot)
(Western blot analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M Lane 2: K562 Lane 3: Rat testis Lane 4: Mouse testis Lane 5: Mouse colon)
Related Product Information for anti-PSMA1 antibody
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Product Categories/Family for anti-PSMA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
263
NCBI Official Full Name
Proteasome subunit alpha type-1
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, alpha type, 1
NCBI Official Symbol
PSMA1
NCBI Official Synonym Symbols
NU; HC2; PROS30; HEL-S-275
NCBI Protein Information
proteasome subunit alpha type-1; PROS-30; protein P30-33K; proteasome nu chain; macropain subunit C2; macropain subunit nu; proteasome subunit nu; 30 kDa prosomal protein; proteasome component C2; proteasome subunit, alpha-type, 1; epididymis secretory pr
UniProt Protein Name
Proteasome subunit alpha type-1
UniProt Gene Name
PSMA1
UniProt Synonym Gene Names
HC2; NU; PROS30; PSC2; PROS-30
UniProt Entry Name
PSA1_HUMAN
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Product Notes
The PSMA1 psma1 (Catalog #AAA30459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Proteasome 20S C2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Proteasome 20S C2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PSMA1 psma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteasome 20S C2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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