<
Rabbit ILF3 Monoclonal Antibody | anti-ILF3 antibody
ILF3 Antibody
Gene Names
ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ILF3, Antibody; ILF3 Antibody; CBTF antibody; Double stranded RNA binding protein 76 antibody; Double-stranded RNA-binding protein 76 antibody; DRBF antibody; DRBP76 antibody; ILF3 antibody; ILF3_HUMAN antibody; Interleukin enhancer binding factor 3 antibody; Interleukin enhancer-binding factor 3 antibody; M phase phosphoprotein 4 antibody; M-phase phosphoprotein 4 antibody; MPHOSPH4 antibody; MPP4 antibody; NF AT 90 antibody; NF-AT-90 antibody; NF110 antibody; NF90 antibody; NFAR antibody; Nuclear factor associated with dsRNA antibody; Nuclear factor of activated T cells 90 kDa antibody; Nuclear factor of activated T-cells 90 kDa antibody; TCP80 antibody; Translational control protein 80 antibody; anti-ILF3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG37-63
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ILF3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein of N-terminal human ILF3.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with ILF3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining ILF3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ILF3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining ILF3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ILF3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-ILF3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ILF3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF3 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1, 000 dilution.)
Related Product Information for anti-ILF3 antibody
ILF3 (interleukin enhancer binding factor 3) or TCP80 (translational control protein 80), is a ubiquitously expressed nuclear protein that exists in a heterodimer with NF45. NF90 contains two DRBM (double-stranded RNA (dsRNA-)-binding) domains and one DZF domain and, in association with NF45 primarily participates in the regulation of IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). NF45 and NF90 belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. Due to alternative splicing events, six isoforms exist for NF90, namely NFAR-2 (or ILF3-E), NFAR-1 (or DRBP76), isoform 3, DRBP76alpha (or ILF3-A), DRBP76delta (also known as DRBP76gamma or ILF3-C) and isoform 6.
Product Categories/Family for anti-ILF3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
894
NCBI Official Full Name
Interleukin enhancer-binding factor 3
NCBI Official Synonym Full Names
interleukin enhancer binding factor 3, 90kDa
NCBI Official Symbol
ILF3
NCBI Official Synonym Symbols
CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
NCBI Protein Information
interleukin enhancer-binding factor 3; M-phase phosphoprotein 4; translational control protein 80; dsRNA binding protein NFAR-2/MPP4; nuclear factor associated with dsRNA; double-stranded RNA-binding protein, 76 kD; nuclear factor of activated T-cells 90
UniProt Protein Name
Interleukin enhancer-binding factor 3
UniProt Gene Name
ILF3
UniProt Synonym Gene Names
DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
UniProt Entry Name
ILF3_HUMAN
