Rabbit anti-Human, Mouse IQGAP1 Monoclonal Antibody | anti-IQGAP1 antibody

IQGAP1 Antibody

Cat. Num. AAA30507

• Gene Names: IQGAP1; SAR1; p195; HUMORFA01
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: IQGAP1, Antibody; IQGAP1 Antibody; HUMORFA01 antibody; IQ motif containing GTPase activating protein 1 antibody; IQGA1_HUMAN antibody; Iqgap1 antibody; KIAA0051 antibody; p195 antibody; Ras GTPase activating like protein 1 antibody; Ras GTPase-activating-like protein IQGAP1 antibody; RasGAP-like with IQ motifs antibody; SAR1 antibody; anti-IQGAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: JG38-74
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-IQGAP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC/IF: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein corresponding to N-terminal human IQGAP1.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of SiHa cells with IQGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining IQGAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining IQGAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining IQGAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IQGAP1 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: HUVEC Lane 2: A431 Lane 3: Mouse placenta)

Related Product Information for anti-IQGAP1 antibody

IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.

Product Categories/Family for anti-IQGAP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506787
• NCBI GeneID: 8826
• NCBI Accession #: NP_003861.1
• NCBI GenBank Nucleotide #: NM_003870.3
• UniProt Accession #: P46940; A7MBM3
• Molecular Weight: 189,252 Da
• NCBI Official Full Name: ras GTPase-activating-like protein IQGAP1
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 1
• NCBI Official Symbol: IQGAP1
• NCBI Official Synonym Symbols: SAR1; p195; HUMORFA01
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
• UniProt Protein Name: Ras GTPase-activating-like protein IQGAP1
• UniProt Gene Name: IQGAP1
• UniProt Synonym Gene Names: KIAA0051
• UniProt Entry Name: IQGA1_HUMAN