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Application Data
Mouse anti-Human CA IX Monoclonal Antibody | anti-CAIX antibody
CA IX Monoclonal Antibody (12F10)
Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
CA IX; Monoclonal Antibody; CA IX Monoclonal Antibody (12F10); Carbonic anhydrase 9; CA9; G250; MN; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1; anti-CAIX antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
12F10
Specificity
The antibody detects endogenous CA IX proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-CAIX antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
IF: 1:50-200
WB: 1:3000
IP: 1:200
IHC: 1:50-300
WB: 1:3000
IP: 1:200
IHC: 1:50-300
Immunogen
Synthetic Peptide of CA IX
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
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WB (Western Blot)
(Western blot analysis of lysates from 1) Hela. 2) 293T cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Tubulin ? Polyclonal Antibody (Assay Biotech:C0380) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of lysates from 1) Hela. 2) 293T cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. Dylight 800 secondary antibody(Assay Biotech:SA0438)was diluted at 1:10000. 37 degree 1hour. (Red) Tubulin ? Polyclonal Antibody (Assay Biotech:C0380) antibody was diluted at 1:5000 as loading control. 4 degree over night.Dylight 680 secondary antibody(Assay Biotech:SA0437)was diluted at 1:10000. 37 degree 1hour.)
WB (Western Blot)
(Western blot analysis of 1) Hela. 2) 293T. diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
WB (Western Blot)
(Western blot analysis of 1) Hela. 2) 293T. diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003.Inventbiotech.MN.USA).)
Related Product Information for anti-CAIX antibody
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Product Categories/Family for anti-CAIX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,698 Da
NCBI Official Full Name
carbonic anhydrase IX
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250
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Product Notes
The CAIX ca9 (Catalog #AAA30813) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CA IX Monoclonal Antibody (12F10) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CA IX can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). IF: 1:50-200 WB: 1:3000 IP: 1:200 IHC: 1:50-300. Researchers should empirically determine the suitability of the CAIX ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA IX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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