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IF (Immunofluorescence)
(Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Mouse Ubiquitin Monoclonal Antibody | anti-RPS27A antibody
Ubiquitin Mouse Monoclonal Antibody (5F1)
Gene Names
RPS27A; UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80
Reactivity
Human, Rat, Mouse, Pig
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Ubiquitin; Monoclonal Antibody; Ubiquitin Mouse Monoclonal Antibody (5F1); anti-RPS27A antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Pig
Clonality
Monoclonal
Clone Number
5F1
Specificity
Ubiquitin protein detects endogenous levels of Ubiquitin
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RPS27A antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000-2000
IHC: 1:100-200
IF: 1:200
IHC: 1:100-200
IF: 1:200
Immunogen
Synthetic Peptide of Ubiquitin
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)
WB (Western Blot)
(Western blot analysis of 1) Hela Cell Lysate. 2) 3T3 Cell Lysate. 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.)
WB (Western Blot)
(Western blot analysis of 1) Hela Cell Lysate. 2) 3T3 Cell Lysate. 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.)
Related Product Information for anti-RPS27A antibody
UBB (ubiquitin B) encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBB consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of UBB are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-RPS27A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
156
NCBI Official Full Name
Ubiquitin-40S ribosomal protein S27a
NCBI Official Synonym Full Names
ribosomal protein S27a
NCBI Official Symbol
RPS27A
NCBI Official Synonym Symbols
UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80
NCBI Protein Information
ubiquitin-40S ribosomal protein S27a; ubiquitin C; ubiquitin-CEP80; epididymis luminal protein 112; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80
UniProt Protein Name
Ubiquitin-40S ribosomal protein S27a
UniProt Gene Name
RPS27A
UniProt Synonym Gene Names
UBA80; UBCEP1
UniProt Entry Name
RS27A_HUMAN
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Product Notes
The RPS27A rps27a (Catalog #AAA30830) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin Mouse Monoclonal Antibody (5F1) reacts with Human, Rat, Mouse, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000-2000 IHC: 1:100-200 IF: 1:200. Researchers should empirically determine the suitability of the RPS27A rps27a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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