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IHC (Immunohistchemistry-Paraffin)
(AAA31217 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit TBLR1 Polyclonal Antibody | anti-TBL1XR1 antibody
TBLR1 Antibody
Gene Names
TBL1XR1; C21; DC42; IRA1; MRD41; TBLR1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TBLR1; Polyclonal Antibody; TBLR1 Antibody; C21; DC42; F box like/WD repeat containing protein TBL1XR1; F-box-like/WD repeat-containing protein TBL1XR1; FLJ12894; IRA1; Nuclear receptor corepressor/HDAC3 complex subunit; Nuclear receptor corepressor/HDAC3 complex subunit TBLR1; TBL1 related protein 1; TBL1-related protein 1; TBL1R_HUMAN; TBL1XR1; Transducin (beta) like 1 X linked receptor 1; Transducin beta like 1X related protein 1; Transducin beta-like 1X-related protein 1; anti-TBL1XR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
TBLR1 Antibody detects endogenous levels of total TBLR1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
514
Applicable Applications for anti-TBL1XR1 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human TBLR1, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistchemistry-Paraffin)
(AAA31217 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistchemistry-Paraffin)
(AAA31217 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry-Paraffin)
(AAA31217 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry-Paraffin)
(AAA31217 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry-Paraffin)
(AAA31217 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry-Paraffin)
(AAA31217 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry-Paraffin)
(AAA31217 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry-Paraffin)
(AAA31217 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using TBLR1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using TBLR1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using TBLR1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 100 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using TBLR1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 100 kDa.)
Related Product Information for anti-TBL1XR1 antibody
Function: F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.
Subcellular Location: Nucleus.
Tissue Specificity: Widely expressed including the pituitary, hypothalamus, white and brown adipose tissue, muscle and liver.
Subunit Structure: Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2 (PubMed:11931768). Probable component of some E3 ubiquitin ligase complex. Interacts with histones H2B and H4 (PubMed:12628926). Interacts with MECP2; bridges interaction between MECP2 and NCOR1 (By similarity).
Similarity: The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.Belongs to the WD repeat EBI family.
Subcellular Location: Nucleus.
Tissue Specificity: Widely expressed including the pituitary, hypothalamus, white and brown adipose tissue, muscle and liver.
Subunit Structure: Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2 (PubMed:11931768). Probable component of some E3 ubiquitin ligase complex. Interacts with histones H2B and H4 (PubMed:12628926). Interacts with MECP2; bridges interaction between MECP2 and NCOR1 (By similarity).
Similarity: The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.Belongs to the WD repeat EBI family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)55kD, 100kD.
Predicted Molecular Weight: (Calculated)56kDa.
Predicted Molecular Weight: (Calculated)56kDa.
NCBI Official Full Name
F-box-like/WD repeat-containing protein TBL1XR1 isoform 1
NCBI Official Synonym Full Names
TBL1X receptor 1
NCBI Official Symbol
TBL1XR1
NCBI Official Synonym Symbols
C21; DC42; IRA1; MRD41; TBLR1
NCBI Protein Information
F-box-like/WD repeat-containing protein TBL1XR1
UniProt Protein Name
F-box-like/WD repeat-containing protein TBL1XR1
UniProt Gene Name
TBL1XR1
UniProt Synonym Gene Names
IRA1; TBLR1
UniProt Entry Name
TBL1R_HUMAN
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Product Notes
The TBL1XR1 tbl1xr1 (Catalog #AAA31217) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TBLR1 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's TBLR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the TBL1XR1 tbl1xr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBLR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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