Sheep Anti-Albumin Antibody ELISA Kit | AAA elisa kit

Sheep Anti-Albumin Antibody ELISA Kit

Cat. Num. AAA93148

• Gene Names: ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
• Reactivity: Sheep
• Synonyms: Anti-Albumin Antibody; N/A; Sheep Anti-Albumin Antibody ELISA Kit; AAA elisa kit

• Reactivity: Sheep
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.

• Intended Uses: This AAA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep AAA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for AAA elisa kit

Principle of the Assay: AAA ELISA kit applies the competitive enzyme immunoassay technique utilizing Anti-Albumin antigen and an AAA-HRP conjugate. The assay sample and buffer are incubated together with AAA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AAA concentration since AAA from samples and AAA-HRP conjugate compete for the Anti-Albumin antigen binding site. Since the number of sites is limited, as more sites are occupied by AAA from the sample, fewer sites are left to bind AAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AAA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 178344
• NCBI GeneID: 213
• Molecular Weight: 47,360 Da
• NCBI Official Full Name: albumin
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Official Synonym Symbols: FDAH; ANALBA; PRO0883; PRO0903; PRO1341
• NCBI Protein Information: serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42
• UniProt Protein Name: Serum albumin
• UniProt Gene Name: ALB
• UniProt Synonym Gene Names: GIG20; GIG42; PRO0903; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341
• UniProt Entry Name: ALBU_HUMAN