Standard Curve (Sample)
Mouse Medium-chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit | Acadm elisa kit
Mouse Medium-chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit
Gene Names
Acadm; MCAD; AU018656
Reactivity
Mouse
Synonyms
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; N/A; Mouse Medium-chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit; MCAD; Acadm; 1.3.8.7; Acadm elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse MCAD. No significant cross-reactivity or interference was observed.
Sequence Length
421
Assay Type
Sandwich
Detection Range
0.312-20 ng/mL
Sensitivity
0.156 ng/mL
Intra-Assay CV
<=6.1%
Inter-Assay CV
<=8.7%
Recovery
105%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Acadm elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse MCAD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCAD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCAD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MCAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCAD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCAD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MCAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MCAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,481 Da
NCBI Official Full Name
medium-chain specific acyl-CoA dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
acyl-Coenzyme A dehydrogenase, medium chain
NCBI Official Symbol
Acadm
NCBI Official Synonym Symbols
MCAD; AU018656
NCBI Protein Information
medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Gene Name
Acadm
UniProt Synonym Gene Names
MCAD
UniProt Entry Name
ACADM_MOUSE
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Product Notes
The Mouse Acadm acadm (Catalog #AAA194205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA194205 ELISA Kit recognizes Mouse Acadm. It is sometimes possible for the material contained within the vial of "Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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