Application Data
(The effect of 0.5ug/ml Mycoplasma Removal Agent (AAA50607) added to the culture medium of mycoplasma-infected cells before (left) and after 1 week (right))
MYCOPLASMA REMOVAL AGENT Accessory Reagent
MYCOPLASMA REMOVAL AGENT
Applications
TC (Tissue Culture)
Synonyms
MYCOPLASMA REMOVAL AGENT; N/A; MYCOPLASMA REMOVAL AGENT accessory reagent
Concentration
Approximate Protein Concentration: 50ug/ml (varies by lot)
Applicable Applications for MYCOPLASMA REMOVAL AGENT accessory reagent
TC (Tissue Culture)
Intended Use
MRA is very effective at removing mycoplasma from infected cell cultures. It shows strong anti-mycoplasma activity against many types of mycoplasma including Mycoplasma orale, M. arginini, M.hyorhinis and Acholeplasma laidlawii.MRA is also suitable for use after the removal of mycoplasma, to prevent recontamination of the culture with the original mycoplasma, at preventative doses.This product can also be used to prevent initial infection of cells in culture by mycoplasma.MRA is non toxic, and will not interfere with the viability or function of cells in culture. It should be emphasised that MRA should not be used as a substitute for good cell culture techniques.
Instructions for Use
MRA is very easy to use, simply requiring incubation for a week after addition to cell cultures contaminated by mycoplasma.
Indications for Use
1. Add MRA to cell cultures contaminated by mycoplasma at a concentration of 0.5 mug/ml and incubate for a week.
2. For media replacement or culture transfer (passage), use a medium containing MRA at this same concentration.
3. Transfer the cell cultures several times without MRA and confirm that regrowth of the contaminating mycoplasma has not occurred.
If there is a concern about the presence of mycoplasma in serum or trypsin, MRA can be added to the media at a concentration of 0.5 mug/ml to prevent contamination of the cell cultures exposed to these products.
N.B. The recommended concentration for use is 0.5 mug/ml. The MRA concentration may be raised up to 1 mug/ml only when the recommended concentration is ineffective in removing the mycoplasma.
The cytotoxicity of MRA is low and cell toxicity is rare when used at the recommended concentration. For specific function of any cell, however, it is recommended that the retention of desired cellular characteristics be confirmed after treatment.
2. For media replacement or culture transfer (passage), use a medium containing MRA at this same concentration.
3. Transfer the cell cultures several times without MRA and confirm that regrowth of the contaminating mycoplasma has not occurred.
If there is a concern about the presence of mycoplasma in serum or trypsin, MRA can be added to the media at a concentration of 0.5 mug/ml to prevent contamination of the cell cultures exposed to these products.
N.B. The recommended concentration for use is 0.5 mug/ml. The MRA concentration may be raised up to 1 mug/ml only when the recommended concentration is ineffective in removing the mycoplasma.
The cytotoxicity of MRA is low and cell toxicity is rare when used at the recommended concentration. For specific function of any cell, however, it is recommended that the retention of desired cellular characteristics be confirmed after treatment.
Preparation and Storage
Store at room temperature.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Application Data
(The effect of 0.5ug/ml Mycoplasma Removal Agent (AAA50607) added to the culture medium of mycoplasma-infected cells before (left) and after 1 week (right))
Application Data
(The effect of 0.5ug/ml Mycoplasma Removal Agent (AAA50607) added to the culture medium of mycoplasma-infected cells before (left) and after 1 week (right))
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Product Notes
The MYCOPLASMA REMOVAL AGENT (Catalog #AAA50607) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MYCOPLASMA REMOVAL AGENT can be used in a range of immunoassay formats including, but not limited to, TC (Tissue Culture). Researchers should empirically determine the suitability of the MYCOPLASMA REMOVAL AGENT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYCOPLASMA REMOVAL AGENT, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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