Application Data
Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) Accessory Reagent
Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa)
Synonyms
Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa); N/A; Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent
Form/Format
62.5mM Tris-H3PO4 (pH7.5, 25°C), 1mM EDTA , 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Features
• Molecular weight range - 10-250 kDa
• Brightness reference band -70 kDa, 25kDa
• Ready to use - Pre-mixed with loading buffer.
• Narrow band
• Brightness reference band -70 kDa, 25kDa
• Ready to use - Pre-mixed with loading buffer.
• Narrow band
Introductions
Prestained protein ladders/marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. It is composed of prestained recombinant prokaryotic proteins. Prestained Protein Molwcular Weight Marker is a three-color molecular weight standard , contains 9 prestained natural proteins with molecular weights from 10kDa to 250kDa. It has two orange reference bands -70kDa band and 25kDa band.
Protocol
1: Thaw the ladder either at room tempeture or at 37°C for a few minutes to disolve precipitated solids thoroughly, Do not boil!
2: Mix gently, but thoroughly to ensure that the solution is homogenous.
3: Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 ul per well, with a thickness of 0.75-1.0 mm.
Large SDS-polyacrylamide gel: 10 ul per well, with thickness of 0.75-1.0 mm.
2: Mix gently, but thoroughly to ensure that the solution is homogenous.
3: Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 ul per well, with a thickness of 0.75-1.0 mm.
Large SDS-polyacrylamide gel: 10 ul per well, with thickness of 0.75-1.0 mm.
Preparation and Storage
Store at 4 degree C for 3 months, and at -20 degree C for long term.
Application Data
Application Data
Related Product Information for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent
Background/Introduction: Polyphenol oxidase is a bifunctional, copper-containing oxidase having catecholase and cresolase activity. It is responsible for browning reactions through the phylogenetic scale. The assay is initiated with the enzymatic hydrolysis of the Catechol by Polyphenol oxidase. The enzyme catalysed reaction products quinone, can be measured at a colorimetric readout at 410 nm.
Product Categories/Family for Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) accessory reagent
Similar Products
Product Notes
The Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa) (Catalog #AAA59055) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Prestained Dual Color Protein Molecular Weight Marker (10-250 kDa), Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.