Rabbit Angiotensin II Converting Enzyme ELISA Kit | ACE II elisa kit

Rabbit Angiotensin II Converting Enzyme ELISA Kit

Cat. Num. AAA85578

• Gene Names: ACE2; ACEH; DKFZp434A014
• Reactivity: Rabbit
• Synonyms: Angiotensin II Converting Enzyme; N/A; Rabbit Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme (Rabbit); ACE II elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACE2. No significant cross-reactivity or interference between ACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Intended Uses: This ACE-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ACE-2. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for ACE II elisa kit

Principle of the Assay: ACE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACE2 antibody and an ACE2-HRP conjugate. The assay sample and buffer are incubated together with ACE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACE2 concentration since ACE2 from samples and ACE2-HRP conjugate compete for the anti-ACE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACE2 from the sample, fewer sites are left to bind ACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ACE2. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for ACE II elisa kit

Rabbit ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 11225609
• NCBI GeneID: 59272
• NCBI Accession #: NP_068576.1
• NCBI GenBank Nucleotide #: NM_021804.2
• UniProt Accession #: Q9BYF1; Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6
• Molecular Weight: 92,463 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• NCBI Official Symbol: ACE2
• NCBI Official Synonym Symbols: ACEH; DKFZp434A014
• NCBI Protein Information: angiotensin-converting enzyme 2; OTTHUMP00000022963; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog
• UniProt Protein Name: Angiotensin-converting enzyme 2
• UniProt Gene Name: ACE2
• UniProt Entry Name: ACE2_HUMAN