Standard Curve (Sample)
Chicken A Disintegrin And Metalloprotease 10 ELISA Kit | ADAM10 elisa kit
Chicken A Disintegrin And Metalloprotease 10 ELISA Kit
Gene Names
ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
Reactivity
Chicken
Synonyms
A Disintegrin And Metalloprotease 10; N/A; Chicken A Disintegrin And Metalloprotease 10 ELISA Kit; ADAM10 elisa kit
Reactivity
Chicken
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Intended Uses
This ADAM10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ADAM10. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ADAM10 elisa kit
Principle of the assay: ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from samples and ADAM10-HRP conjugate compete for the anti-ADAM10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM10 from the sample, fewer sites are left to bind ADAM10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM10 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ADAM10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
84,142 Da
NCBI Official Full Name
ADAM10
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
ADAM10
NCBI Official Synonym Symbols
RAK; kuz; AD10; AD18; MADM; CD156c; HsT18717
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10; CDw156; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Gene Name
ADAM10
UniProt Synonym Gene Names
KUZ; MADM; ADAM 10
UniProt Entry Name
ADA10_HUMAN
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Product Notes
The Chicken ADAM10 adam10 (Catalog #AAA89309) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA89309 ELISA Kit recognizes Chicken ADAM10. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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