Application Data
A Disintegrin And Metalloprotease 28 (ADAM28) Recombinant Protein | ADAM28 recombinant protein
Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)
Gene Names
ADAM28; MDCL; MDC-L; ADAM23; MDC-Lm; MDC-Ls; eMDCII; ADAM 28; eMDC II
Applications
Immunoprecipitation, Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
>98%
Synonyms
A Disintegrin And Metalloprotease 28 (ADAM28); N/A; Recombinant A Disintegrin And Metalloprotease 28 (ADAM28); ADAM28 recombinant protein
Host
E Coli
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-EVADKS CYNRNEGGSKYGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMVANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSSVVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVKAVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK
MGHHHHHHSGSEF-EVADKS CYNRNEGGSKYGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMVANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSSVVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVKAVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK
Sequence Length
775
Applicable Applications for ADAM28 recombinant protein
IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Glu525-Lys774 (Accession # Q9UKQ2) with N-terminal His-Tag
Tissue Specificity
Spleen, Stomach, Intestine, Brain.
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Traits
Freeze-dried powder
Predicted isoelectric point
7.5
Predicted Molecular Mass
29.3kDa
Accurate Molecular Mass
34kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Sequence
Sequence
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
disintegrin and metalloproteinase domain-containing protein 28 isoform 1 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 28
NCBI Official Symbol
ADAM28
NCBI Official Synonym Symbols
MDCL; MDC-L; ADAM23; MDC-Lm; MDC-Ls; eMDCII; ADAM 28; eMDC II
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 28; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 28
UniProt Gene Name
ADAM28
UniProt Synonym Gene Names
ADAM23; MDCL; ADAM 28; eMDC II; MDC-L
UniProt Entry Name
ADA28_HUMAN
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Product Notes
The ADAM28 adam28 (Catalog #AAA134290) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's A Disintegrin And Metalloprotease 28 (ADAM28) can be used in a range of immunoassay formats including, but not limited to, IP (CoImmunoprecipitation), IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the ADAM28 adam28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-EVADKS CYNRNEGGSK YGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMV ANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSS VVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVK AVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 28 (ADAM28), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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