Human ADAM metallopeptidase with ombospondin type 1 motif ELISA Kit | ADAMTS13 elisa kit
Human ADAM metallopeptidase with ombospondin type 1 motif, 13 (ADAMTS13) ELISA Kit
Reactivity
Human
Synonyms
ADAM metallopeptidase with ombospondin type 1 motif; N/A; Human ADAM metallopeptidase with ombospondin type 1 motif, 13 (ADAMTS13) ELISA Kit; ADAMTS13 elisa kit
Reactivity
Human
Sequence Length
1935
Assay Type
Sandwich
Detection Range
50 ng/mL-0.78 ng/mL
Sensitivity
Up to 0.5 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for ADAMTS13 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human ADAMTS13 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
213,420 Da
NCBI Official Full Name
ADAM metallopeptidase with thrombospondin type 1 motif, 9
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 9
NCBI Official Symbol
ADAMTS9
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 9
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 9
UniProt Gene Name
ADAMTS9
UniProt Synonym Gene Names
KIAA1312; ADAM-TS 9; ADAM-TS9; ADAMTS-9
UniProt Entry Name
ATS9_HUMAN