Human A disintegrin and metalloproteinase with thrombospondin motifs 8 ELISA Kit | ADAMTS8 elisa kit
Human A disintegrin and metalloproteinase with thrombospondin motifs 8 ELISA Kit
Gene Names
ADAMTS8; METH2; ADAM-TS8
Reactivity
Human
Synonyms
A disintegrin and metalloproteinase with thrombospondin motifs 8; N/A; Human A disintegrin and metalloproteinase with thrombospondin motifs 8 ELISA Kit; ADAMTS8/A disintegrin and metalloproteinase with thrombospondin motifs 8/ADAM-TS 8/ADAM-TS8/ADAMTS-8/METH-2/METH-8/METH2/ADAM metallopeptidase with thrombospondin type 1 motif; 8/ADAM-TS8A disintegrin and metalloproteinase with thrombospondin motifs 8; ADAMTS8 elisa kit
Reactivity
Human
Specificity
Specifically recognize ADAMTS8, no obvious cross reaction with other analogues
Sequence Length
889
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for ADAMTS8 elisa kit
Background: ADAMTS-8, also designated METH-2, METH-8 or FLJ41712, along with ADAMTS-1 (METH-1, C3-C5), represent a new family of proteins with metalloprotease, disintegrin and Thrombospondin domains. ADAMTS-8 is highly expressed in adult and fetal lung tissue while detected at lower levels in heart, placenta, stomach, brain and kidney tissue. The spacer region and the Thrombospondin type I motifs in the carboxy-terminus of ADAMTS-8 are important for anchoring the protein to the extracellular matrix.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ADAMTS8 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ADAMTS8 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ADAMTS8 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ADAMTS8 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti ADAMTS8 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti ADAMTS8 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with ADAMTS8 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of ADAMTS8 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,460 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 8 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 8
NCBI Official Symbol
ADAMTS8
NCBI Official Synonym Symbols
METH2; ADAM-TS8
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 8
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 8
UniProt Gene Name
ADAMTS8
UniProt Synonym Gene Names
METH2; ADAM-TS 8; ADAM-TS8; ADAMTS-8
UniProt Entry Name
ATS8_HUMAN
