Standard Curve (Sample)
Human adipose differentiation-related protein ELISA Kit | ADFP elisa kit
Human adipose differentiation-related protein, ADRP ELISA Kit
Gene Names
PLIN2; ADFP; ADRP
Reactivity
Human
Synonyms
adipose differentiation-related protein; N/A; Human adipose differentiation-related protein, ADRP ELISA Kit; Human adipose differentiation-related protein; ADRP ELISA Kit; RP11-151J10.1; ADFP; ADRP; MGC10598; adipophilin; ADFP elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ADFP. No significant cross-reactivity or interference between Human ADFP and analogues was observed.
Sequence Length
437
Samples
Serum, urine, cell culture supernates, tissue homogenates
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
0.78 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ADFP elisa kit
Introduction: Adipose differentiation-related protein, is a protein which in humans is encoded by the ADRP gene. Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADRP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADRP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADRP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADRP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,075 Da
NCBI Official Full Name
perilipin-2
NCBI Official Synonym Full Names
perilipin 2
NCBI Official Symbol
PLIN2
NCBI Official Synonym Symbols
ADFP; ADRP
NCBI Protein Information
perilipin-2; adipophilin; adipose differentiation-related protein
UniProt Protein Name
Perilipin-2
UniProt Gene Name
PLIN2
UniProt Synonym Gene Names
ADFP; ADRP
UniProt Entry Name
PLIN2_HUMAN
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Product Notes
The Human ADFP plin2 (Catalog #AAA80708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80708 ELISA Kit recognizes Human ADFP. It is sometimes possible for the material contained within the vial of "adipose differentiation-related protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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