SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Adiponectin (globular domain) Recombinant Protein | Adipoq recombinant protein
Adiponectin (globular domain), 111-247aa, Mouse, E Coli
Gene Names
Adipoq; Ad; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Adiponectin (globular domain); N/A; Adiponectin (globular domain), 111-247aa, Mouse, E Coli; ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin globular domain; Mouse; Adipocyte; C1q and collagen domain-containing protein; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; Adipose most abunda; Adiponectin; 30 kDa adipocyte complement related protein; ACRP 30; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipose most abundant gene transcript 1; APM 1; GBP 28; Gelatin binding protein,; Adipoq recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT, 10% glycerol.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN
Sequence Length
111-247
Applicable Applications for Adipoq recombinant protein
SDS-PAGE
Tag
Non-Tagged
Domain
111-247aa
Endotoxin Level
<1 EU per 1ug of protien (determined by LAL method)
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for Adipoq recombinant protein
Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30 (amino acid residues, 111-247, gAcrp30) was overexpressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Adipoq recombinant protein
References
Joachim Fruebis, et al (2001) PNAS 98 (4) 2005-2010; Das,K., et al (2001) Biochem. Biophys.Res.Commun. 280 (4)1120-1129; Yamauchi T., et al (2001) Nature Medicine 7 (8) 941-946; Berg AH, et al (2001) Nature Medicine 7 (8) 947-952; Yamauchi. T., et al (2002) Nature Medicine 8 (11) 1288-1295;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16 kDa (138 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
Adipoq
NCBI Official Synonym Symbols
Ad; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30
NCBI Protein Information
adiponectin
UniProt Protein Name
Adiponectin
UniProt Gene Name
Adipoq
UniProt Synonym Gene Names
Acdc; Acrp30; Apm1; ACRP30
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Product Notes
The Adipoq adipoq (Catalog #AAA48453) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adiponectin (globular domain) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Adipoq adipoq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN. It is sometimes possible for the material contained within the vial of "Adiponectin (globular domain), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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